[PMC free article] [PubMed] [Google Scholar]Sheff DR, Daro EA, Hull M, Mellman I

[PMC free article] [PubMed] [Google Scholar]Sheff DR, Daro EA, Hull M, Mellman I. Golgi to the basolateral membrane were all relatively unaffected by Rac1V12 manifestation. Rac1N17 manifestation experienced little or no effect on these postendocytic or biosynthetic trafficking pathways. These results display that in polarized MDCK cells triggered Rac1 may regulate the BAY 61-3606 dihydrochloride pace BAY 61-3606 dihydrochloride of endocytosis from both membrane domains and that manifestation of dominating active Rac1V12 specifically alters postendocytic and biosynthetic membrane traffic directed to the apical, but not the basolateral, membrane. Intro The compartmentalized nature of the eukaryotic cell requires a highly regulated protein trafficking system to establish and maintain membrane, organellar, and cellular identity (Mostov and Cardone, 1995 ). As a result, the quality and quantity of biosynthetic and endocytic traffic must be capable of changing in response to extracellular signals. The Rho family of small GTPases represents an important class of molecules that can modify cellular functions in response to a variety of extracellular signals (Vehicle Aelst and D’Souza-Schorey, 1997 ). This family currently comprises at least seven users and their isoforms (Mackay and Hall, 1998 ): Rho (A and B isoforms), Rac (1 and 2 isoforms), Cdc42 (Cdc42Hs and G25K isoforms), RhoD, RhoG, RhoE, and TC10. In the beginning, Rho family members were shown to regulate the formation of specialized actin constructions in the cell: RhoA settings the formation of stress materials and focal adhesion (Ridley and Hall, 1992 ), Rac1 directs lamellipodia formation (Ridley by RhoA (Adam by Cdc42 (Chen stained over night with 0.5% (wt/vol) uranyl acetate in H2O. Filters were dehydrated inside a graded series of ethanol, inlayed in the epoxy resin LX-112 (Ladd, Burlington, VT), and sectioned having a diamond knife (Diatome, Fort Washington, PA). Sections, pale platinum in color, were mounted on butvar-coated nickel grids and viewed at 80 kV inside a (Japan) 100 CX electron microscope without further contrasting. Endocytosis of [125I]IgA Endocytosis of [125I]IgA was measured as explained (Breitfeld (1996) but are in contrast to those IGFBP2 of Li (1997) , who found no effect of dominating active Rac1 manifestation on fluid-phase endocytosis in baby BAY 61-3606 dihydrochloride hamster kidney cells. This discrepancy may reflect variations in cell type or the use of a fluid phase marker by Li and ligands that undergo receptor-mediated endocytosis in our study and that of Lamaze access into epithelial cells. EMBO J. 1996;15:3315C3321. [PMC free article] [PubMed] [Google Scholar]Adamson P, Paterson HF, Hall A. Intracellular localization of the p21rho proteins. J Cell Biol. BAY 61-3606 dihydrochloride 1992;119:617C627. [PMC free article] [PubMed] [Google Scholar]Annaert WG, Becker B, Kistner U, Reth M, Jahn R. Export of cellubrevin from your endoplasmic reticulum is definitely controlled by BAP31. J Cell Biol. 1997;139:1397C1410. [PMC free article] [PubMed] [Google Scholar]Apodaca G, Aroeti B, Tang K, Mostov KE. Brefeldin-A inhibits the delivery of the polymeric immunoglobulin receptor to the basolateral surface of MDCK cells. J Biol Chem. 1993;268:20380C20385. [PubMed] [Google Scholar]Apodaca G, Katz LA, Mostov KE. Receptor-mediated transcytosis of IgA in MDCK cells is definitely via apical recycling endosomes. J Cell Biol. 1994;125:67C86. [PMC free article] [PubMed] [Google Scholar]Aroeti B, Kosen PA, Kuntz ID, Cohen FE, Mostov KE. Mutational and secondary structural analysis of the basolateral sorting transmission of the polymeric immunoglobulin receptor. J Cell Biol. 1993;123:1149C1160. [PMC free article] [PubMed] [Google Scholar]Aroeti B, Mostov KE. Polarized sorting of the polymeric.